PTM Viewer

For each protein all PTMs are highlighted by default in the respective protein sequence (right-hand side). One can adjust a selection of PTMs in the PTM table on the left-hand side. In addition, functional protein domains and sites can be underlined if desired.

In the PTM table per PTM the PTM position and type is indicated, as well as the plain peptide sequence that was identified by mass spectrometry. The respective proteomics study is indicated by a number, providing a link to consult the experimental details. Additional PTM meta-data includes various confidence measures such as peptide score provided by search algorithms, posterior error probability (PEP), precursor mass deviation (in ppm) and modification site probability. The available confidence meta-data can be consulted in the extended PTM table by clicking SHOW CONFIDENCE. However, in the default PTM table, a color-coding of confidence is provided with green indicating high confidence, olive medium confidence, grey low confidence, and no color an unassigned confidence. More details regarding this confidence assignment can be consulted in the tutorial or the Plant PTM Viewer manuscript.

Besides confidence measures, log2 fold changes between two conditions with significance values (P- or Q-values) are shown if provided in the respective publication. Log2 fold changes are colored in heatmap-like gradient (green = induced, red = repressed) and significant values are highlighted in green. To determine significance, we employed the threshold used in the respective publication. For more details on the quantitative measurements we refer to the experimental details and respective publication, as methodologies can differ.

On the bottom of the page one can send the whole protein or a part of the protein (i.e. a functional domain) to PTM Blast. This will display aligned protein sequences that potentially report aligned PTMs.

PTMs

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Results

PTM Type	Mod AA	Pos	Peptide	Exp ID	Log2 FC	P/Q val	Score Type	Ident Score	Loc Prob	PEP	Mass Error (ppm)
nt	M	108	MLLNSVGQDE	99	-0.497	0.110	MASCOT Score	48.780		5.215e-4	-1.351
nt	M	138	METVEPGQTSHER	99			MASCOT Score	36.340		7.290e-2	-1.525
nt	M	138	METVEPGQTSHER	118	4.166	0.000	MaxQuant score	121.740		2.480e-33
nta	M	138	METVEPGQTSHERSLSKEETVNLQPNPSV	167a
			METVEPGQTSHER	80			MASCOT Score	57.280		8.363e-4
			METVEPGQTSHER	99			MASCOT Score	36.340		7.290e-2	-1.525
ph	S	191	TDDGQEQVLVKDDSPTAVEEASVEEK	18a			MaxQuant Score	41.214	0.860	1.000e-3	-0.591
				84a			XCorr	6.880	0.995	9.970e-5
				85			XCorr	5.913	0.999	1.740e-4
				109					0.990
				111a	-0.079	0.000		72.970			0.902
				111b	-0.079	0.000		72.970			0.902
				111c	0.447	0.000		72.970			0.902
				111d	0.447	0.000		72.970			0.902
				136	0.001	0.994	MASCOT score	116.390			-1.485
			DDSPTAVEEASVEEK	88			MASCOT Score	36.000	1.000		0.180
				100			XCorr	4.380	0.987	2.237e-5
				106			MASCOT Score	35.000
				114			MaxQuant score	401.450	1.000	0.000e+0
ph	T	229	IGTETTDNLLDQTEEK	114			MaxQuant score	222.570	0.998	0.000e+0
ph	S	302	SILVEGHSDSHIDSASEVEK	100			XCorr	3.273	0.997	2.558e-4
ph	S	309	SILVEGHSDSHIDSASEVEK	114			MaxQuant score	92.499	0.970	3.520e-7
ph	S	311	SILVEGHSDSHIDSASEVEKVEAENIGK	114			MaxQuant score	205.810	0.840	0.000e+0
ph	S	341	EIELSDVTVLER	114			MaxQuant score	187.740	1.000	2.248e-20
ph	S	476	TSNLEQSMELPVNANDRDQDVK	114			MaxQuant score	57.688	1.000	9.705e-5
ph	S	570	TSSDDSDQGSYSETKK	114			MaxQuant score	139.640	0.965	1.560e-8
ph	S	573	TSSDDSDQGSYSETK	88			MASCOT Score	30.000	0.896		-0.630
ph	S	573	TSSDDSDQGSYSETK	114			MaxQuant score	159.830	1.000	6.740e-14
ph	S	579	TSSDDSDQGSYSETKK	114			MaxQuant score	169.600	0.857	2.730e-19
ph	S	623	QSTYSVEGCPR	114			MaxQuant score	72.681	0.986	2.275e-3
ph	S	707	DGQEEDNTAASGGIMAAGTPVTHPK	114			MaxQuant score	162.550	0.998	0.000e+0
ph	T	715	DGQEEDNTAASGGIMAAGTPVTHPK	100			XCorr	5.681	0.998	4.170e-10
				109					0.930
				114			MaxQuant score	239.930	0.975	0.000e+0
ph	S	730	GDAIVLGDSR	114			MaxQuant score	57.566	1.000	1.839e-2
ph	Y	743	SYVTAIEDAATNLK	100			XCorr	2.806	0.866	2.140e-4
ph	S	760	TPLDSFPTVK	114			MaxQuant score	124.890	1.000	9.293e-5
ph	S	792	KPEDQNISGFMSAGSPVLNR	114			MaxQuant score	122.570	0.853	6.057e-17
ph	S	795	KPEDQNISGFMSAGSPVLNR	85			XCorr	3.952	1.000	4.108e-7
				88			MASCOT Score	73.000	1.000		-0.420
				100			XCorr	5.987	1.000	1.980e-12
				109					1.000
				111a	0.640	0.000		26.880			0.650
				111b	0.640	0.000		26.880			0.650
				111c	0.308	0.000		26.880			0.650
				111d	0.308	0.000		26.880			0.650
				114			MaxQuant score	439.170	1.000	0.000e+0
ph	T	803	NETSSSEMNLTPDQLK	88			MASCOT Score	43.000	0.992		-0.110
ph	T	803	NETSSSEMNLTPDQLK	106			MASCOT Score	41.000
ph	S	804	NETSSSEMNLTPDQLK	88			MASCOT Score	29.000	0.986		0.450
ph	S	804	NETSSSEMNLTPDQLK	114			MaxQuant score	145.360	0.994	7.151e-10
ph	S	805	NETSSSEMNLTPDQLK	85			XCorr	3.528	0.910	3.650e-7
				88			MASCOT Score	43.000	0.983		-0.110
				106			MASCOT Score	41.000
				114			MaxQuant score	307.890	0.997	0.000e+0
ph	S	806	NETSSSEMNLTPDQLK	88			MASCOT Score	35.000	0.990		-2.410
ph	S	806	NETSSSEMNLTPDQLK	114			MaxQuant score	204.070	0.892	0.000e+0
ph	T	811	NETSSSEMNLTPDQLK	88			MASCOT Score	42.000	1.000		-0.220
ph	T	811	NETSSSEMNLTPDQLK	114			MaxQuant score	391.760	1.000	0.000e+0
og	T	830	AVIFSQATLVSPIVVGSPSTSSLDKTAAK	81			MASCOT Score	28.900			2.300
og	S	833	AVIFSQATLVSPIVVGSPSTSSLDKTAAK	81			MASCOT Score	28.900			2.300
ph	T	883	GATPIEQFQSGGK	114			MaxQuant score	143.880	1.000	8.860e-6
ph	S	890	GATPIEQFQSGGK	114			MaxQuant score	143.880	1.000	8.860e-6
og	S	900	GATPIEQFQSGGKTNAVNQSLASHIQITQSTEK	81			MASCOT Score	22.700			4.500
ph	S	919	SLQSPALK	88			MASCOT Score	38.000	1.000		-1.670
ph	S	919	SLQSPALK	114			MaxQuant score	135.860	1.000	2.587e-3
og	S	1008	SLVATPETPLQSRPGKTETPSAGHTNSK	81			MASCOT Score	40.800			0.880
og	T	1012	SLVATPETPLQSRPGKTETPSAGHTNSK	81			MASCOT Score	24.700			1.800
ph	T	1012	SLVATPETPLQSR	88			MASCOT Score	27.000	0.942		-0.650
ph	T	1012	SLVATPETPLQSR	114			MaxQuant score	136.920	1.000	7.686e-6
ph	T	1015	SLVATPETPLQSRPGK	114			MaxQuant score	187.750	1.000	0.000e+0
ph	T	1015	SLVATPETPLQSR	88			MASCOT Score	27.000	1.000		-0.650
og	S	1019	SLVATPETPLQSRPGKTETPSAGHTNSK	81			MASCOT Score	38.500			1.800
og	T	1024	SLVATPETPLQSRPGKTETPSAGHTNSK	81			MASCOT Score	53.100			4.200
fuc	T	1032	SLVATPETPLQSRPGKTETPSAGHTNSK	162						4.000e-1
fuc	S	1034	SLVATPETPLQSRPGKTETPSAGHTNSK	162						7.400e-1
ph	S	1100	GSAATHQPLLSASHAHQTPPTQNIVGHNTPWMSPLPFR	114			MaxQuant score	144.140	1.000	0.000e+0
ph	T	1135	FPVYPITDPVKLTPMK	114			MaxQuant score	114.530	1.000	1.354e-7
ph	S	1141	ESSMTLSGAK	114			MaxQuant score	84.507	0.974	2.069e-3
ph	S	1145	ESSMTLSGAK	114			MaxQuant score	116.130	0.978	1.802e-4
og	S	1152	HVQSGTSSNVSKVTPTLEPTSTVVAPAQHSTR	81			MASCOT Score	48.100			1.400
og	T	1162	VTPTLEPTSTVVAPAQHSTR	81			MASCOT Score	43.900			0.680
og	T	1164	HVQSGTSSNVSKVTPTLEPTSTVVAPAQHSTR	81			MASCOT Score	48.100			1.400
og	S	1169	VTPTLEPTSTVVAPAQHSTR	81			MASCOT Score	35.600			0.680
ph	S	1178	VTPTLEPTSTVVAPAQHSTR	114			MaxQuant score	239.030	0.823	0.000e+0
ph	S	1258	SSFPSPIFGGNLVR	100			XCorr	2.298	1.000	3.902e-3
ph	S	1258	SSFPSPIFGGNLVR	114			MaxQuant score	76.759	0.994	6.329e-4
ph	T	1388	SYDSILPGQGTPASILK	114			MaxQuant score	154.460	0.904	1.062e-15
ox	C	1483	QAQESQEVQPCK	47	0.068	0.491
ph	S	1494	ETVSTSEGTFAGPK	83			MASCOT Score	28.000			-0.001
ph	S	1494	ETVSTSEGTFAGPK	114			MaxQuant score	173.590	0.999	2.216e-17
ph	T	1495	ETVSTSEGTFAGPK	114			MaxQuant score	156.080	0.971	6.374e-10
ph	S	1496	ETVSTSEGTFAGPK	83			MASCOT Score	71.000			-0.000
				85			XCorr	2.727	0.938	2.613e-7
				100			XCorr	4.215	0.823	1.760e-6
				106			MASCOT Score	27.000
				109					0.980
				114			MaxQuant score	137.810	0.997	1.457e-5
ph	T	1499	ETVSTSEGTFAGPK	83			MASCOT Score	25.000			-0.000
ph	S	1642	SVTALPYEGTR	88			MASCOT Score	37.000	0.994		0.680
				109					0.941
				114			MaxQuant score	160.670	0.996	1.082e-10
ph	S	1734	WIECSSSGETISSSHEGDTPK	114			MaxQuant score	312.010	0.979	0.000e+0
ph	T	1741	WIECSSSGETISSSHEGDTPKEK	114			MaxQuant score	180.650	0.999	0.000e+0
ph	T	1751	LGTPALVAEVK	88			MASCOT Score	61.000	1.000		-0.040
				100			XCorr	2.706	1.000	7.377e-4
				114			MaxQuant score	108.590	1.000	9.704e-5
ub	K	1854	KEPVKPVR	40			MaxQuant Score	97.635	1.000	7.000e-3	0.547
ph	S	1864	SIVPQNSGIGSWK	114			MaxQuant score	122.100	0.994	6.526e-5
ph	S	1868	SIVPQNSGIGSWK	114			MaxQuant score	104.550	0.966	1.783e-4
ac	K	1870	SIVPQNSGIGSWKMPSKTISIEK	98b	4.341	0.105	MaxQuant Score	77.700	1.000	1.000e-11
ac	K	1874	SIVPQNSGIGSWKMPSKTISIEK	98b	4.341	0.105	MaxQuant Score	77.700	1.000	1.000e-11
ph	S	1918	NTTASDMESDESAENR	88			MASCOT Score	57.000	0.997		0.110
ph	S	1918	NTTASDMESDESAENR	114			MaxQuant score	174.520	1.000	1.424e-19
ph	S	1922	NTTASDMESDESAENRGPGSGVSFK	83			MASCOT Score	34.000			-0.001
			NTTASDMESDESAENR	88			MASCOT Score	67.000	1.000		-1.010
			NTTASDMESDESAENR	114			MaxQuant score	173.250	1.000	0.000e+0
ph	S	1925	NTTASDMESDESAENRGPGSGVSFK	83			MASCOT Score	34.000			-0.001
			NTTASDMESDESAENR	88			MASCOT Score	67.000	1.000		-1.010
			NTTASDMESDESAENR	114			MaxQuant score	181.720	1.000	0.000e+0
ph	S	1947	GTVEEQTTSSSHDTGSK	114			MaxQuant score	166.300	0.846	5.751e-22
ph	S	2028	IPSVSHSKSHLSQSK	114			MaxQuant score	76.118	0.765	1.086e-4
ph	S	2030	IPSVSHSKSHLSQSK	114			MaxQuant score	119.500	0.914	1.124e-6

Sequence

Length: 2037

MDYDDSDFQNQNLHLAGEANNKFPPVLQPYALPKFDFDDTLNTHLRFDSLGESEAFLGIEGNEDNNWIEDFSRGSSGIVFSSGATESCAISRHNNVWSEATSSESVAMLLNSVGQDEVIVREDTIKKSDTSHELGCTMETVEPGQTSHERSLSKEETVNLQPNPSVDDTPGESSVVKTDDGQEQVLVKDDSPTAVEEASVEEKNSILTSNTATVEAIDTTDLGKIGTETTDNLLDQTEEKANVESRMEDDCSDGNVQTIITCSGELNNQSTLLPETSNDENVISDHIQSSYNRNDLTADARSILVEGHSDSHIDSASEVEKVEAENIGKTAKPDLKEIELSDVTVLERGDQAPSTLEVGGQDVSGTECQDLLVSTVHTSVAVEASLELAGELTTITNSVSIEKPELLSHQHMEVITSEHESTFQIETETYPQIHVFETSESVYISTMDSMVEAREGGVSKKSDNEGSARTSNLEQSMELPVNANDRDQDVKNSQILSESVVSGSVGYVSGGSTSELAESESQSDSIPTDKSETMIDSSLNLEELQPLSQDGAPAVSLTSSIDLHMVKTSSDDSDQGSYSETKKVYGEPENGQTVPPVDASCSGSQMDQEARKRAEGTKQSTYSVEGCPRSEGSKDAVDADGVGQVLQQQSEELIFEENVVTEAVKAPETLSVLDKDNKNEMPITSSLPILGSEAGKDGQEEDNTAASGGIMAAGTPVTHPKGDAIVLGDSRASTCSESSVKSYVTAIEDAATNLKTPLDSFPTVKTSELQFNNTETNSVKKPEDQNISGFMSAGSPVLNRNETSSSEMNLTPDQLKAGKISKAVIFSQATLVSPIVVGSPSTSSLDKTAAKSSKAKSERKPRRTSKSVGKETSRKGTSVKGATPIEQFQSGGKTNAVNQSLASHIQITQSTEKQRSLQSPALKAFGSLSTPTASLPDLNSSALSSILRRPFTDLQQVQLRAQIFVYGALIQGTAPDEAYMISAFGGADGGKGSWEKSWRTCVVRAQKSLVATPETPLQSRPGKTETPSAGHTNSKESSGTNPMIPLSSPLWSLSTSVDTLQSSSVQRGSAATHQPLLSASHAHQTPPTQNIVGHNTPWMSPLPFRNAWLASQQTSGFDVGSRFPVYPITDPVKLTPMKESSMTLSGAKHVQSGTSSNVSKVTPTLEPTSTVVAPAQHSTRVKSRKRKKMPVSVESGPNILNSLKQTELAASPLVPFTPTPANLGYNAGTLPSVVSMTAVPMDLVSTFPGKKIKSSFPSPIFGGNLVREVKQRSVLSEDTIEKLKEAKMHAEDASALATAAVSHSEYVWKQIEQQSHAGLQPETQDRLASAAVAIAAAAAVAKAAAAAANVAANAALQAKLMAEEASLPNASDQGLPKSYDSILPGQGTPASILKGEGAVVNSSSVLIAAREASKKRVEAATAATKRAENMDSIVKAAELASEAVSQAGILVSMGHPPLLNKLVEAGPSNYWRQAQESQEVQPCKTVVLEKETVSTSEGTFAGPKIVQTEFGGSVNTADGVSGPVPATGKLKGQEGDKYADLAKNNDVVFEPEVGSKFSIDAQQTIKATKNEDIKEGSNVEVFKEEPGLRTAWYSANVLSLEDDKAYVLFSDLSVEQGTDKLKEWVALKGEGDQAPKIRPARSVTALPYEGTRKRRRAALGDHIWKIGDRVDSWVHDSWLEGVITEKNKKDENTVTVHFPAEEETLTIKAWNLRPSLVWKDGKWIECSSSGETISSSHEGDTPKEKRPRLGTPALVAEVKDTSMKIVDDPDLGKPPQTGVLNLGVSENTFNIGKSTREENKPDPLRMKRTGLQKQGSKVIFGVPKPGKKRKFMDVSKHYVSEASTKTQERKEPVKPVRSIVPQNSGIGSWKMPSKTISIEKQTTISRPKTFKPAPKPKEKPGATARIIPRKDSRNTTASDMESDESAENRGPGSGVSFKGTVEEQTTSSSHDTGSKNSSSLSTNKGRVAPTAGRLAKIEEDKALAENSSKTSEGMEPRRSIRRIQPTSRLLEGLQTSMMTSKIPSVSHSKSHLSQSKK

ID	PTM Type	Color
nt	N-terminus Proteolysis	X
nta	N-terminal Acetylation	X
ph	Phosphorylation	X
og	O-GlcNAcylation	X
fuc	O-Fucosylation	X
ox	Reversible Cysteine Oxidation	X
ub	Ubiquitination	X
ac	Acetylation	X
	Multiple types	X

Domains & Sites

Clear highlighted range

**Interpro Domains**
IPR ID	Description	From	To
IPR008395	Agenet-like domain	1576	1640
IPR014002	Agenet domain, plant type	1571	1635
IPR014002	Agenet domain, plant type	1662	1720

AT4G17330.1

Arabidopsis thaliana [ath]

G2484-1 protein

71 PTM sites : 8 PTM types

PLAZA: AT4G17330

Gene Family: HOM05D003141

Other Names: ATG2484-1

Uniprot

F4JP43

Link out to other resources with this protein ID : TAIR | PeptideAtlas | ARAPORT | PhosPhAt

PTMs

Sequence

Length: 2037

Domains & Sites

BLAST

Perform a BLAST search for this sequence, or a part of this sequence (minimum 50 characters)
A downloadable tutorial can be found here

AT4G17330.1

Arabidopsis thaliana [ath]

G2484-1 protein

71 PTM sites : 8 PTM types

PLAZA: AT4G17330

Gene Family: HOM05D003141

Other Names: ATG2484-1

Uniprot

F4JP43

Link out to other resources with this protein ID : TAIR | PeptideAtlas | ARAPORT | PhosPhAt

PTMs

Sequence

Length: 2037

Domains & Sites

BLAST

Perform a BLAST search for this sequence, or a part of this sequence (minimum 50 characters)A downloadable tutorial can be found here

Perform a BLAST search for this sequence, or a part of this sequence (minimum 50 characters)
A downloadable tutorial can be found here